Inscrição na biblioteca: Guest

ISSN Online: 2377-424X

ISBN CD: 1-56700-226-9

ISBN Online: 1-56700-225-0

International Heat Transfer Conference 13
August, 13-18, 2006, Sydney, Australia

REAL-TIME POLYMERASE CHAIN REACTION CONTROLLED BY JOULE HEATING EFFECT IN ELECTROKINETICALLY-DRIVEN MICROCHIP

Get access (open in a dialog) DOI: 10.1615/IHTC13.p14.40
9 pages

Resumo

While electrokinetic means can greatly simplify species transport in microfluidic lab-on-chip devices, a significant drawback is the internal Joule heating generated by current flow through the buffer solution. In this work, a new method to control Polymerase Chain Reaction (PCR) thermal cycling in a microchannel using Joule heating effect was presented. Joule heating was generated internally by the current flow through the buffer solution in an electrokinetically-driven microfluidic chip without external heater component. Theoretic model was developed and numerical simulations were conducted to determine the parameters required to achieve the desired thermal cycling. A PDMS-based microchannel chip was designed and fabricated. The power consumption was only 1.3 W for such a Joule heating-controlled PCR chip. To demonstrate the feasibility of using Joule heating to control thermal cycling, a DNA fragment DNA of Escherichia coli O157:H7 stx1 was successfully amplified by a two-temperature TaqMan real-time polymerase chain reaction. The peak power consumption was only 1.3 W. There is still much room to improve the thermal response of Joule heating if the total heat capacitance of the chip can be reduced.