As global warming intensifies due to the use of fossil fuels, many countries around the world are implementing eco-friendly policies by promoting carbon neutrality. Hydrogen is regarded as the future fuel source for a zero-carbon society based on its zero-emission properties. Among hydrogen storage strategies, liquid hydrogen is actively investigated due to its high volumetric energy density and low storage pressure. Since liquid hydrogen has very low normal boiling point, which is about 20 K, the storage tank needs high insulation performance. In order to construct efficient insulation designs, understanding of heat transfer, especially through multi-layer insulation (MLI) is important. In this study, an integrated thermal modeling of liquid hydrogen storage tank is developed by MATLAB to analyze the effects of various MLI designs on heat transfer, hydrogen storage mass, dormancy, and boil-off rate characteristics. The model consists of three parts; tank design, heat transfer, dynamic two-phase hydrogen. The tank design part is composed of inner/outer shell, MLI, suspension, fill/drain pipe, vent line, vaporizer, and level sensor design. A thermal network model considering that Lockheed equation is designed for the heat transfer part, and dynamic two-phase hydrogen part is modelled using mass balance and energy balance. Using the integrated model, the effects of MLI thickness, number of layers, spacer materials on heat transfer rate, hydrogen storage mass, dormancy, and boil-off rate are investigated. The results show that as the MLI thickness increases, not only the heat transfer rate, but also the hydrogen storage mass is reduced. Also, there is an optimum point of the heat transfer rate depending on the MLI number of layers. In addition, silk net as a spacer of MLI shows the best insulation performance among various MLI spacer materials. It is expected that an optimal MLI design can be developed by referring to these results.