As one of the most common phenomena in nature, droplet evaporation has become a major heat transfer mode and is used widely in industries and scientific fields. For a sessile droplet, the wettability of the substrate surface could make a significant effect on evaporation dynamics and heat transfer rate. Among many factors influencing surface wettability, surface roughness is one of the common causes, but the link between roughness and the processes of droplet evaporation has not yet been fully revealed. Therefore, an experimental study of sessile nanofluid water droplets located on copper surfaces with different roughness and wettability has been carried out. In the present study, the effect of roughness as a factor on the evaporation rate is considered. The deformation and temperature distribution of the liquid-vapour interface of the sessile droplet are recorded. In addition, the effect of surface roughness on the nanoparticle sedimentary pattern is observed. It has been demonstrated that the changes in surface roughness not only affect the wetting state of the sessile droplet but also have an impact on the heat and mass transfer processes at the liquid-vapour interface. The research in this article aims to recognize the relationship between roughness and droplet evaporation dynamics.